Will the Typhoon system do ethidium bromide?
A. Yes. Molecular Dynamics do not currently provide sensitivity data for the Typhoon, however the Fluoroimager system is about 5 times more sensitive for ethidium bromide visualization than the traditional UV light box and Polaroid film setup. VISTRA Green, a DNA dye available fromAmersham Pharmacia Biotech, is used the same way as ethidium bromide and offers 100 times greater sensitivity than the traditional ethidium bromide method. See Molecular Dynamics’ Application note 64 – Fluorescent DNA Gel Stain Detection for further information and a protocol.
