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dige

Question tag: dige 25 Questions

0 Votes 1 Ans

Are there differences in labelling between the three CyDye™ DIGE Fluor minimal dyes?

cydye™ differences dige dyes fluor labelling

0 Votes 1 Ans

How does the staining pattern differ between CyDye™ DIGE Fluor minimal dyes, fluorescent post-labelling and silver-stained gels?

cydye™ differ dige dyes fluor fluorescent Pattern staining

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Why can’t I pick directly from a gel containing CyDye™ DIGE Fluor labelled proteins?

containing cydye™ dige directly fluor gel labelled pick Proteins

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Is the IEF of protein samples labelled with CyDye™ DIGE Fluor minimal dyes more difficult or technically demanding?

cydye™ difficult dige dyes fluor ief labelled protein samples

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Does labelling the proteins with CyDye™ DIGE Fluor minimal dyes affect the isoelectric focusing parameters?

affect cydye™ dige dyes fluor focusing isoelectric labelling Proteins

0 Votes 1 Ans

How many different sample types have been analyzed using the CyDye™ DIGE Fluor minimal dye labelling approach?

analyzed cydye™ different dige dye fluor labelling sample types

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Why can’t I use commercially available plastic backed precast gels with CyDye™ DIGE Fluor minimal dyes?

cydye™ dige fluor gels plastic precast

0 Votes 1 Ans

When imaging the SYPRO™ Ruby stained gel, can I still image for the original CyDye™ DIGE Fluor minimal dyes and get three (or four) related images?

cydye™ dige fluor gel image imaging original ruby stained sypro™

0 Votes 1 Ans

When imaging a SYPRO™ Ruby stained gel, can I still image for the original CyDye™ DIGE Fluor minimal dyes and get three (or four) related images?

cydye™ dige fluor gel image imaging original ruby stained sypro™

0 Votes 1 Ans

added onto a protein after labelling with each CyDye™ DIGE Fluor minimal dye?

added cydye™ dige dye fluor labelling protein

0 Votes 1 Ans

CyDye™ DIGE Fluor Cy™2, Cy3 and Cy5 minimal dyes have different quantum yields. Does this effect the accuracy with which protein abundance changes can be quantified?

cy3 cy5 cydye™ different dige dyes fluor Quantum

0 Votes 1 Ans

Do the CyDye™ DIGE Fluor minimal dyes cause suppression in MS ionization?

cause cydye™ dige dyes fluor ionization MS suppression

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How long will my CyDye™ DIGE Fluor minimal dyes keep once I have reconstituted them?

cydye™ dige dyes fluor reconstituted

0 Votes 1 Ans

Can I use Destreak Reagent to improve the resolution of basic proteins when using 2-D DIGE?

BASIC dige Proteins Reagent resolution

0 Votes 1 Ans

What protein concentration range is recommended prior to the start of labelling with CyDye™ DIGE Fluor minimal dyes?

concentration cydye™ dige fluor labelling prior protein range recommended

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What is the general process of 2D DIGE?

0 Votes 1 Ans

What is the turnaround time for 2D DIGE?

dige time turnaround

0 Votes 1 Ans

How can I do pathway analysis after the 2D DIGE/protein ID experiment?

analysis dige experiment Id Pathway protein

0 Votes 1 Ans

How do I prepare immunoprecipitated (IP) samples for 2D DIGE?

dige immunoprecipitated IP prepare samples

0 Votes 1 Ans

What is the sensitivity of 2D DIGE? How many protein spots can you detect in the 2D DIGE experiment?

detect dige experiment protein spots