Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Does labelling the proteins with CyDye™ DIGE Fluor minimal dyes affect the isoelectric focusing parameters?

April 26, 2017affect cydye™ dige dyes fluor focusing isoelectric labelling Proteins

0

Posted

Does labelling the proteins with CyDye™ DIGE Fluor minimal dyes affect the isoelectric focusing parameters?

1 Answer

0

Posted

We recommend that a high salt compatible isoelectric focusing program be used to focus proteins labelled with CyDye DIGE Fluor minimal dyes (refer to the Ettan DIGE System User Manual for examples of focusing protocols). CyDye DIGE Fluor minimal dyes are designed to ensure that the net charge of the protein remains unchanged upon labelling so the isoelectric point is unaffected. Focusing times should likewise remain unchanged.