How does the Coomassie Plus Protein Assay work?
The Coomassie Plus Protein Assay is based on an absorbance shift from 465 nm to 595 nm that occurs when the reagent binds to proteins in an acidic solution. The mechanism of the reaction is the anionic form of the dye complexes with proteins, resulting in a color change from brown to blue. The dye interacts chiefly with arginine residues and weakly with histidine, lysine, tyrosine, tryptophan and phenylalanine residues. The reaction reaches a stable endpoint so absorbance does not shift over time.
