What is Coomassie Plus Protein Assay Reagent?
Pierce Coomassie Plus Protein Assay Reagent is a quick and ready-to-use modification of the well-known Bradford, Coomassie dye-binding, colorimetric method for total protein quantitation.1 This Bradford reagent modification greatly reduces the tendency of Coomassie dye-containing reagents to give a characteristically nonlinear response curve. Coomassie Plus Reagent results in a substantially more linear response curve in a defined range of protein concentration. Pierce’s special formulation results in significantly less protein-to-protein variation than that which is observed with other Bradford-type Coomassie dye-based formulations. When Coomassie dye binds protein in an acidic medium, an immediate absorbance shift occurs from 465 nm to 595 nm, with a simultaneous color change of the reagent from green/brown (or red/brown) to blue.
