My samples have too much antibody from the immunoprecipitation. How can I get rid of it?
There are a couple of possible strategies to reduce the amount of antibody in the IP elution. One is to use competitive elution if possible, for example using antigenic peptide (FLAG, Myc, are examples). Another is to use covalently bound antibody. If you are working with a construct with a His-tag, the last step could be metal column instead of an antibody.
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