What is the minimum number of cells required to perform a Roche NimbleGen recommended ChIP-chip experiment?
The minimum number of cells used for a successful ChIP-chip experiment is approximately 1 x 107. However, ChIP reactions in which abundant molecules are being immunoprecipitated (e.g. histones and RNA polymerase II) require a lesser number of cells for a successful experiment. Remember, if you are starting with less material you would need to adjust all of the volumes and concentrations in each protocol.
