When MA Lipofection Enhancer is used does the mixing order of components influence the final transfection efficiency?
All commercial transfection reagents can be associated with MA Lipofection Enhancer reagent by simple mixing in order to generate magnetic delivery systems. Depending on the transfection reagent used, the mixing order of components may influence the final transfection efficiency of MATra. We recommend to use 1 µl of MA Lipofection Enhancer per µg of DNA in initial experiments (for antisense oligos or siRNA ratios need to be optimized) and to prepare the DNA / transfection reagent complexes according to the reagent’s manufacturer instructions first and then add the nanoparticles. The usual final incubation step after mixing DNA & the commercial transfection reagent can be omitted by adding directly the magnetic particles into the mixture. However, depending on the cell line to be transfected and the commercial transfection reagent used, the optimal composition may require the optimization of the MA Lipofection Enhancer/DNA ratio.
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